ICAR has developed India’s first fully annotated telomere-to-telomere (T2T) reference genome of pigeonpea variety ‘Asha’

ICAR’s fully annotated Telomere-to-Telomere (T2T) genome of pigeonpea ‘Asha’—now the global reference genome—marks a major milestone in Indian agricultural genomics, enabling faster development of climate-resilient, high-yielding, and nutrient-rich pulse varieties while advancing India’s goal of self-reliance in pulses.

Historic Achievement

  • ICAR developed India’s first fully annotated Telomere-to-Telomere (T2T) reference genome.
  • Crop: Pigeonpea (Cajanus cajan L.)
  • Variety: ‘Asha’
  • Approved as the global reference genome for pigeonpea.

Why Pigeonpea Matters

  • Popularly known as Arhar/Tur
  • One of India’s most important pulse crops
  • Major source of plant protein
  • Critical for:
    • Food security
    • Nutritional security
    • Farmer livelihoods

What is a T2T Genome?

Telomere-to-Telomere (T2T) Genome

A complete DNA sequence covering an organism’s chromosomes from one telomere to the other, including previously difficult-to-sequence regions.

Advantages

  • Highest genome accuracy
  • Complete chromosome map
  • Better gene discovery
  • Supports precision breeding & genome editing

Genome Highlights

  • 752.65 million base pairs
  • 92 contigs
  • Complete assembly of 11 chromosomes
  • Includes:
    • 11 centromeres
    • 22 telomeres
  • Genome Assembly:
    • NIPB_CcT2T_4

Gene Annotation

Researchers identified:

  • 36,557 genes
  • 48,008 mRNAs (coding sequences)
  • >99.9% RNA read alignment
    • Confirms high accuracy & completeness

Global Recognition

  • Genome deposited in NCBI global database.
  • Accession No.:
    • GCF_000230855.1
  • Officially released:
    • 20 April 2026

Why This Breakthrough Matters

The genome will accelerate:

  • Gene discovery
  • Pangenome research
  • Molecular breeding
  • Genome editing
  • Trait identification

Focus Traits

  • Climate resilience
  • Higher yield
  • Disease & stress tolerance
  • Better nutritional quality

🌾 Benefits for Agriculture

  • Faster development of improved pigeonpea varieties
  • Increased pulse productivity
  • Reduced import dependence
  • Improved farmer income
  • Stronger national food & nutritional security

🏛️ Building on Earlier Milestones

ICAR–National Institute for Plant Biotechnology (NIPB)

  • 2011
    • World’s first draft genome of a pulse crop
    • First crop genome sequenced entirely in India
  • 2017
    • Improved draft genome released
  • 2026
    • Complete Telomere-to-Telomere (T2T) reference genome

Role of ICAR

ICAR supports pulse development through:

  • High-yielding varieties
  • Pest-resistant varieties
  • Climate-resilient breeding
  • Breeder seed production
  • Multi-location trials
  • Krishi Vigyan Kendras (KVKs)
  • Farmer training & technology dissemination

Mission ‘Aatmanirbharta in Pulses’

Duration: 2025–26 to 2030–31

Objectives

  • Achieve pulse self-sufficiency
  • Reduce imports
  • Increase farmer income
  • Expand improved seed adoption
  • Strengthen procurement & post-harvest infrastructure

Target

  • 35 million tonnes of pulse production by 2030–31